User:Kmcallenberg/Accessible surface area

From Wikipedia, the free encyclopedia
Illustration of the solvent accessible surface in comparison to the van der Waals surface. The van der Waals surface as given by the atomic radii is shown in red. The accessible surface is drawn with dashed lines and is created by tracing the center of the probe sphere (in blue) as it rolls along the van der Waals surface. Note that the probe radius depicted here is of smaller scale than the typical 1.4Å.

The accessible surface area (ASA) is the surface area of a biomolecule that is accessible to a solvent. The ASA is usually quoted in square ångstrom (a standard unit of measurement in molecular biology). ASA was first described by Lee & Richards in 1971 and is sometimes called the Lee-Richards molecular surface.[1] ASA is typically calculated using the 'rolling ball' algorithm developed by Shrake & Rupley in 1973.[2] This algorithm uses a sphere (of solvent) of a particular radius to 'probe' the surface of the molecule.

Methods of calculating ASA[edit]

Shrake-Rupley algorithm[edit]

The Shrake-Rupley method draws a mesh of points equidistant from each atom of the molecule and uses the number of these points that are solvent accessible to determine the surface area.[2] The points are drawn at a water molecule's estimated radius beyond the van der Waals radius, which is effectively similar to ‘rolling a ball’ along the surface. All points are checked against the surface of neighboring atoms to determine whether they are buried or accessible. The number of points accessible is multiplied by the portion of surface area each point represents to calculate the ASA. The choice of the 'probe radius' does have an effect on the observed surface area, as using a smaller probe radius detects more surface details and therefore reports a larger surface. A typical value is 1.4Å, which approximates the radius of a water molecule. Another factor that affects the results is the definition of the VDW radii of the atoms in the molecule under study. For example, the molecule may often lack hydrogen atoms which are implicit in the structure. The hydrogen atoms may be implicitly included in the atomic radii of the 'heavy' atoms, with a measure called the 'group radii'. In addition, the number of points created on the van der Waals surface of each atom determines another aspect of discretization, where more points provide an increased level of detail.

LCPO method[edit]

The LCPO method uses a linear approximation of the two-body problem for a quicker analytical calculation of ASA.[3] The approximations used in LCPO result in an error in the range of 1-3 Ų.

Applications[edit]

Accessible surface area is often used when calculating the transfer free energy required to move a biomolecule from aqueous solvent to a non-polar solvent such as a lipid environment. The LCPO method is also used when calculating implicit solvent effects in the molecular dynamics software package AMBER.

Similar surfaces[edit]

The ASA is closely related to the concept of the solvent-excluded surface (also known as the molecular surface or Connolly surface), which is imagined as a cavity in bulk solvent (effectively the inverse of the solvent-accessible surface). It is also calculated in practice via a rolling-ball algorithm developed by Frederic Richards[4] and independently implemented three-dimensionally by Michael Connolly in 1983[5] and Tim Richmond in 1984.[6] Connolly spent several more years perfecting the method.[7]

See also[edit]

Notes[edit]

  1. ^ Lee B, Richards FM. (1971). The interpretation of protein structures: estimation of static accessibility. J Mol Biol 55(3):379-400. doi:10.1016/0022-2836(71)90324-X
  2. ^ a b Shrake A, Rupley JA. (1973). Environment and exposure to solvent of protein atoms. Lysozyme and insulin. J Mol Biol 79(2):351-71. doi:10.1016/0022-2836(73)90011-9
  3. ^ Weiser J, Shenkin PS, Still WC (1999). "Approximate atomic surfaces from linear combinations of pairwise overlaps (LCPO)". Journal of Computational Chemistry. 20 (2): 217-230. doi:10.1002/(SICI)1096-987X(19990130)20:2<217::AID-JCC4>3.0.CO;2-A.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  4. ^ Richards FM. (1977). "Areas, volumes, packing and protein structure." Annu Rev Biophys Bioeng, 6:151–176. doi:10.1146/annurev.bb.06.060177.001055
  5. ^ M. L. Connolly. "Analytical molecular surface calculation." J Appl Cryst, 16(5):548–558, Oct 1983. doi:10.1107/S0021889883010985
  6. ^ T. J. Richmond. "Solvent accessible surface area and excluded volume in proteins. Analytical equations for overlapping spheres and implications for the hydrophobic effect." J Mol Biol, 178(1):63–89, Sep 1984. doi:10.1016/0022-2836(84)90231-6
  7. ^ M. L. Connolly. "The molecular surface package." J Mol Graphics, 11(2):139-141, Oct 1992. doi:10.1016/0263-7855(93)87010-3

References[edit]

  • Connolly,M.L. "solvent-accessible surfaces of proteins and nucleic-acids" (1983) Science, 221, 709–713
  • Connolly, M.L. et al. "Molecular interstitial skeleton," 1991.
  • Sanner, M.F. et al. "Modelling and Applications of Molecular Surfaces," PhD, 1992.
  • Connolly, M.L. et al. "Shape distributions of protein topography," 1992.
  • Blaney, J. M. et al. "Distance Geometry in Molecular Modeling," 1994.
  • Boissonnat, J.-D. et al. "Computing Connolly Surfaces," 1994
  • Connolly, M.L. et al. "Molecular Surfaces: A Review," 1996.
  • Lin, S. L. et al. "Molecular surface representations by sparse critical points," 1994.
  • Connolly, M.L. et al. "Molecular Surfaces: Calculations, Uses and Representations," 2001.
  • Gerstein, M & Richards, F.S. "Protein Geometry: Distances, Areas, and Volumes," 2001
  • Voss, N.R. et al. "The Geometry. of the Polypeptide Exit Tunnel," 2006
  • Leach, A. "Molecular Modelling: Principles and Applications," 2001, 2nd ed., Page 7

External links[edit]

Category:Molecular modelling Category:Computational chemistry Category:Molecular dynamics Category:Protein structure

Retrieved from "https://en.wikipedia.org/w/index.php?title=User:Kmcallenberg/Accessible_surface_area&oldid=1118582009"