File:Neisseria-meningitidis-Differentially-Controls-Host-Cell-Motility-through-PilC1-and-PilC2-pone.0006834.s003.ogv

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Neisseria-meningitidis-Differentially-Controls-Host-Cell-Motility-through-PilC1-and-PilC2-pone.0006834.s003.ogv ‎(Ogg Theora video file, length 22 s, 300 × 225 pixels, 974 kbps, file size: 2.5 MB)

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Description
English: Live-imaging of ME180 cells infected with strain NmPilC1ind. ME180 cells were infected with meningococcal strains NmPilC1ind (PilC1ind/PilC2-) that expresses pilC1 under the control of an IPTG-inducible promoter. Cells were monitored continuously before infection (in the absence of IPTG), after addition of infecting bacteria (MOI 50, no IPTG), and after the induction of PilC1 expression by addition of IPTG to the medium. Adhesion to ME180 cells relies on the expression of PilC1, upon IPTG-mediated induction. However, cellular motility is reduced in the case of PilC1-mediated infection, whereas it remains unaffected in the case of PilC2-mediated adhesion (Video S2).
Date
Source Video S1 from Morand P, Drab M, Rajalingam K, Nassif X, Meyer T (2009). "Neisseria meningitidis Differentially Controls Host Cell Motility through PilC1 and PilC2 Components of Type IV Pili". PLOS ONE. DOI:10.1371/journal.pone.0006834. PMID 19718432. PMC: 2729722.
Author Morand P, Drab M, Rajalingam K, Nassif X, Meyer T
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current21:58, 30 October 201222 s, 300 × 225 (2.5 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.
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