Talk:ADP-ribosylation

A fact from ADP-ribosylation appeared on Wikipedia's Main Page in the Did you know column on 16 September 2007. The text of the entry was as follows:

Did you know... that ADP-ribosylation, a posttranslational protein modification, is involved in the actions of several bacterial toxins in diseases such as cholera and whooping cough?

A record of the entry may be seen at Wikipedia:Recent additions/2007/September.

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Molecular Biology: MCB

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"Mechanistic figure needs a change"[edit]

I propose that the mechanism provided in this page be modified - it appears that the carboxy groups rather than the side chains of the glutamate residues are engaged in contacts with the Arg and the ribose (Step 2). See Lüscher et al. Chemical Reviews 2017. Kpuvar25 (talk) 17:48, 19 December 2019 (UTC)[reply]

Mechanism seems inaccurate[edit]

The mechanistic figure and associated text does not seem to be factual, or is at least dubious. There is no citation describing how the glutamic acid residues deprotonate the arginine, as described in the text. Furthermore, investigation into this class of enzymes (i.e., EXE motif ADP ribosyltransferases) suggests that the glutamic acid residues are "...thought to stabilize the furanosyl oxocarbenium intermediate" (Cohen and Chang, Nat Chem Biol., 2018 Feb 14). See also Tsurumura et al., PNAS, 2013 March 12, which supports this role of the catalytic glutamic acid residues, as well as Tsuge et al., PNAS, 2008 May 27, which shows a similar (but not equivalent) mechanism as described in the figure/text. Furthermore, the glutamic acid side chain carboxyl groups seem to be mediating catalysis, not the amino acid carboxyl group--the latter would not be present due to being part of the peptide backbone and would therefore exist as a peptide bond. The text and mechanistic figure are thus in need of proper citation, or a removal of the proposed mechanism altogether.Hakuran90 (talk) 20:05, 25 November 2020 (UTC)[reply]

Damned fools[edit]

If the mechanism isn’t to your liking, draw a new figure and replace it. Suggesting catalytic alternative is useless. 2603:8001:6901:812A:54C7:B98A:CE7:45D7 (talk) 18:27, 20 October 2022 (UTC)[reply]