File:Sanger-sequencing.svg
Size of this PNG preview of this SVG file: 673 × 463 pixels. Other resolutions: 320 × 220 pixels | 640 × 440 pixels | 1,024 × 704 pixels | 1,280 × 881 pixels | 2,560 × 1,761 pixels.
Original file (SVG file, nominally 673 × 463 pixels, file size: 2.67 MB)
 | This is a file from the Wikimedia Commons. Information from its description page there is shown below. Commons is a freely licensed media file repository. You can help. |
Summary
| Description |
English: The Sanger (chain-termination) method for DNA sequencing. (1) A primer is annealed to a sequence, (2) Reagents are added to the primer and template, including: DNA polymerase, dNTPs, and a small amount of all four dideoxynucleotides (ddNTPs) labeled with fluorophores. During primer elongation, the random insertion of a ddNTP instead of a dNTP terminates synthesis of the chain because DNA polymerase cannot react with the missing hydroxyl. This produces all possible lengths of chains. (3) The products are separated on a single lane capillary gel, where the resulting bands are read by a imaging system. (4) This produces several hundred thousand nucleotides a day, data which require storage and subsequent computational analysis .
Español: El método de terminación de la cadena de Sanger. (1) Un cebador se alínea a la secuencia, (2) Se añaden reactivos al cebador y ADN molde incluyendo: polimerasa de ADN, dNTPs, una pequeña cantidad de todos los cuatro didesoxinucleótidos (ddNTPs) marcados con fluoróforos. Durante la elongación de los cebadores, una inserción aleatoria de un ddNTP en vez de un dNTP termina la síntesis de la cadena debido a que la ADN polimerasa no puede reaccionar con el hidroxilo faltante. Esto produce todas las posibles longitudes de las cadenas. (3) Los productos se separan en un gel capilar, donde las bandas resultantes se leen por un sistema de imágenes. (4) Esto produce muchos cientos de miles de nucleótidos al día, y los datos requieren almacenaje y posterior análisis por computadora. |
| Date | |
| Source | Own work |
| Author | Estevezj |
Licensing
I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
This file was derived from:
- Laser-symbol.svg:
- Generic-office-desktop2.svg:
- Laser printer isometric.svg:
- Dideoxyadenosine triphosphate.svg:
- Desoxyadenosintriphosphat protoniert.svg:
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 19:55, 20 December 2012 | | 673 × 463 (2.67 MB) | Estevezj | included chemical structures for ddNTPs and dNTPs |
| 07:29, 20 December 2012 |  | 673 × 463 (2.63 MB) | Estevezj | Replaced Sans with Helvetica, deleted wonky layer. | |
| 06:52, 20 December 2012 |  | 650 × 387 (2.3 MB) | Estevezj | Reverted to version as of 06:50, 20 December 2012 bad image | |
| 06:51, 20 December 2012 |  | 671 × 440 (2.64 MB) | Estevezj | English labels. | |
| 06:50, 20 December 2012 |  | 650 × 387 (2.3 MB) | Estevezj | User created page with UploadWizard |
File usage
The following pages on the English Wikipedia use this file (pages on other projects are not listed):
Global file usage
The following other wikis use this file:
- Usage on bs.wikipedia.org
- Usage on en.wikibooks.org
- Usage on fa.wikipedia.org
- Usage on fi.wikipedia.org
- Usage on gl.wikipedia.org
- Usage on ko.wikipedia.org
- Usage on ro.wikipedia.org
- Usage on tr.wikipedia.org
