File:Ramos cell trogocytosis.png

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Description
English: Volumetric reconstruction from confocal slices of a Ramos-RAW cell interface. RTX-Al488-coated (green), PKH26-labelled Ramos cells (red) were incubated with RAW cells for 45 minutes at 37°C. RAW cells were labelled with anti-CD11b-APC (cyan). The RAW cell has extensively trogocytosed both RTX and PKH26. Inset shows the dotted area above it without the PKH26 channel overlaid, revealing the concentration of RTX-Al488 at the cell-cell interface, otherwise depleted from the rest of the Ramos cell. Trogocytosis reaction was halted by fixation 45 min after co-incubation. Ramos cells are approximately 12 µm in diameter.
Español: Reconstrucción volumétrica de preparaciones para microscopía confocal de una interfaz entre una célula de Ramos y una célula RAW. Las células de Ramos fueron revestidas con RTX-Al488 (en verde) y marcadas con PKH26 (rojo). Posteriormente se incuban con células RAW durante 45 minutos a 37º. Las células RAW se marcan con anti-CD11b-APC (en cian). La célula RAW ha trogocitado ampliamente tanto el RTX como el PKH26.
Date
Source PLoS One
Author Theodore Pham, Patricia Mero, James W. Booth.

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