File:DNA Nanoball Adapter Ligation.png

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English: The Adapter sequences AD1L and AD1R are ligated to the size selected DNA. The adapters complement each other and the DNA is circularized. A restriction enzyme is added that cleaves the DNA. AD2L and AD2R are ligated, the DNA is circularized and a restriction enzyme cleaves the DNA. AD3L and AD3R are added, the DNA is circularized, and a type III restriction enzyme is added. This cleaves out a 300 - 400bp piece of DNA and AD4 replaces it to complete the circular, adapter ligated template DNA.
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Author Suspencewl
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Date/TimeThumbnailDimensionsUserComment
current19:51, 28 February 2011Thumbnail for version as of 19:51, 28 February 2011902 × 1,026 (542 KB)SuspencewlLower resolution so it can fit in page better as non-thumb
22:58, 24 February 2011Thumbnail for version as of 22:58, 24 February 20111,282 × 1,026 (332 KB)SuspencewlReverted to version as of 22:46, 24 February 2011
22:49, 24 February 2011Thumbnail for version as of 22:49, 24 February 20111,202 × 1,026 (660 KB)SuspencewlThe Adapter sequences AD1L and AD1R are ligated to the size selected DNA. The adapters complement each other and the DNA is circularized. A restriction enzyme is added that cleaves the DNA. AD2L and AD2R are ligated, the DNA is circularized and a restrict
22:46, 24 February 2011Thumbnail for version as of 22:46, 24 February 20111,282 × 1,026 (332 KB)Suspencewl{{Information |Description ={{en|1=The Adapter sequences AD1L and AD1R are ligated to the size selected DNA. The adapters complement each other and the DNA is circularized. A restriction enzyme is added that cleaves the DNA. AD2L and AD2R are ligate
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